Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen.
Identifieur interne : 000924 ( France/Analysis ); précédent : 000923; suivant : 000925Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen.
Auteurs : Xavier Denier [France] ; Elisabeth M. Hill ; Jeanette Rotchell ; Christophe MinierSource :
- Toxicology in vitro : an international journal published in association with BIBRA [ 0887-2333 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Cadmium, Copper, Endocrine Disruptors, Estradiol, Zinc.
- drug effects : Yeasts.
- growth & development : Yeasts.
- Dose-Response Relationship, Drug, Drug Synergism.
Abstract
Heavy metals are increasingly studied due to their apparent ability to disrupt signaling pathways of living organisms including humans. Among various mechanisms of action, metals are suspected of exerting estrogenic activity in human and wildlife. In this study, a wide range of concentration of cadmium, copper, lead, mercury and zinc (from 95.4 pM to 1 mM) alone or in combination with the natural estrogen, 17-beta estradiol, has been tested using the yeast estrogen screen, an estrogen receptor dependent transcriptional expression assay. No direct trans-activation of the estrogen-responsive element could be measured with any of the concentration of the metals tested. Nevertheless, cadmium, copper and zinc were able to potentiate the estradiol-induced response in a dose-dependent manner. Significant stimulation was obtained from 10 nM cadmium, 100 nM copper and 2 nM zinc. Maximum response led to decrease of the estradiol EC50 by a factor 10. This study indicates that cadmium, copper and zinc can act as potential endocrine disrupters by modulating the estrogenic activity of endogenous hormones.
DOI: 10.1016/j.tiv.2009.01.006
PubMed: 19490851
Affiliations:
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pubmed:19490851Le document en format XML
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Hill</name></author><author><name sortKey="Rotchell, Jeanette" sort="Rotchell, Jeanette" uniqKey="Rotchell J" first="Jeanette" last="Rotchell">Jeanette Rotchell</name></author><author><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19490851</idno><idno type="pmid">19490851</idno><idno type="doi">10.1016/j.tiv.2009.01.006</idno><idno type="wicri:Area/PubMed/Corpus">000242</idno><idno type="wicri:Area/PubMed/Curation">000242</idno><idno type="wicri:Area/PubMed/Checkpoint">000242</idno><idno type="wicri:Area/Ncbi/Merge">000233</idno><idno type="wicri:Area/Ncbi/Curation">000233</idno><idno type="wicri:Area/Ncbi/Checkpoint">000233</idno><idno type="wicri:doubleKey">0887-2333:2009:Denier X:estrogenic:activity:of</idno><idno type="wicri:Area/Main/Merge">000B49</idno><idno type="wicri:Area/Main/Curation">000B43</idno><idno type="wicri:Area/Main/Exploration">000B43</idno><idno type="wicri:Area/France/Extraction">000924</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen.</title><author><name sortKey="Denier, Xavier" sort="Denier, Xavier" uniqKey="Denier X" first="Xavier" last="Denier">Xavier Denier</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, University of Le Havre, BP 540, 76058 Le Havre, France. xavier.denier@gmail.com</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology, University of Le Havre, BP 540, 76058 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Hill, Elisabeth M" sort="Hill, Elisabeth M" uniqKey="Hill E" first="Elisabeth M" last="Hill">Elisabeth M. Hill</name></author><author><name sortKey="Rotchell, Jeanette" sort="Rotchell, Jeanette" uniqKey="Rotchell J" first="Jeanette" last="Rotchell">Jeanette Rotchell</name></author><author><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name></author></analytic><series><title level="j">Toxicology in vitro : an international journal published in association with BIBRA</title><idno type="ISSN">0887-2333</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium (pharmacology)</term><term>Copper (pharmacology)</term><term>Dose-Response Relationship, Drug</term><term>Drug Synergism</term><term>Endocrine Disruptors (pharmacology)</term><term>Estradiol (pharmacology)</term><term>Yeasts (drug effects)</term><term>Yeasts (growth & development)</term><term>Zinc (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cadmium</term><term>Copper</term><term>Endocrine Disruptors</term><term>Estradiol</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Yeasts</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Yeasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Dose-Response Relationship, Drug</term><term>Drug Synergism</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Heavy metals are increasingly studied due to their apparent ability to disrupt signaling pathways of living organisms including humans. Among various mechanisms of action, metals are suspected of exerting estrogenic activity in human and wildlife. In this study, a wide range of concentration of cadmium, copper, lead, mercury and zinc (from 95.4 pM to 1 mM) alone or in combination with the natural estrogen, 17-beta estradiol, has been tested using the yeast estrogen screen, an estrogen receptor dependent transcriptional expression assay. No direct trans-activation of the estrogen-responsive element could be measured with any of the concentration of the metals tested. Nevertheless, cadmium, copper and zinc were able to potentiate the estradiol-induced response in a dose-dependent manner. Significant stimulation was obtained from 10 nM cadmium, 100 nM copper and 2 nM zinc. Maximum response led to decrease of the estradiol EC50 by a factor 10. This study indicates that cadmium, copper and zinc can act as potential endocrine disrupters by modulating the estrogenic activity of endogenous hormones.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Hill, Elisabeth M" sort="Hill, Elisabeth M" uniqKey="Hill E" first="Elisabeth M" last="Hill">Elisabeth M. Hill</name><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><name sortKey="Rotchell, Jeanette" sort="Rotchell, Jeanette" uniqKey="Rotchell J" first="Jeanette" last="Rotchell">Jeanette Rotchell</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Denier, Xavier" sort="Denier, Xavier" uniqKey="Denier X" first="Xavier" last="Denier">Xavier Denier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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